MolBioCore

The MolBioCore offers a comprehensive array of equipment and provides technical consultation and support for projects such as DNA cloning to make expression vectors, real-time PCR analysis, recombinant protein production and protein purification. Technical training can also be provided for self-directed equipment use.

  • Designing of DNA construct and DNA cloning to create expression vectors
  • Preparation of plasmid DNA
  • Electrophoresis of protein and DNA samples
  • Western blot analysis
  • Protein/peptide purification using FPLC/ HPLC
  • Production of recombinant protein
  • Quantification of gene expression by real-time PCR
  • Designing and development of gene specific real-time PCR
  • Quantification of hormones and cytokines by immunoassays

  • Technical training    $65/hr

    Highly qualified staff provides technical assistance on procedures and method development related to molecular biology and protein chemistry

    Unassisted Instrumentation Use

    MBioCore uses automated, high quality instruments to produce efficient and reliable results.

    Centrifugation

    Avanti J-26 XP/XP1 High performance  

    $7 / hour 

    J6 MI High Capacity 

    $7 / hour 

    Optima L-100XP Ultracentrifuge 

    $14 /hour

    Plate Readers

    EnVision 2104  $6 / plate 
    MSD SECTOR Imager 6000 multiplex  $11 / plate 

    Plate Washer

     Skan Washer 400 $3 / use

    PCR

    Applied Biosystems 7900HT Realtime  $14 / use

    BioRad T-100 Thermal Cycler 

    $ 4 / use

    DNA/RNA/Protein Quantification

    Nanodrop 1000/3000  $2 / use 

    Imaging

     GE ImageQuant $2 / photo
    GE Typhoon Trio $10 / scan 
    GE InCell Analyzer 6000  $20 / plate
     Olympus OV110 Small animal imager $55 / hour

    Alberta Diabetes Institute members receive 25% off price list

    Equipment Access and Use 

    Monday–Friday from 9.00–18.00

  • Mandatory training for all first-time users
  •  to book an appointmentkunimasa@ualberta.caEmail Kuni Suzuki (Core Manager) 
  • Security card access available for users
  • Instruments must remain free of debris, spills or damage following use
    Note: Cleaning/repair costs will be charged back to user
  • Molecular biology protocols are diverse and experience counts when it comes to developing protocols that bring you results with high quality. MolBioCore has extensive experience optimizing protocols and providing assistance on its equipment. 

    MOLBIOCORE

    Alberta Diabetes Institute
    6-030 Li Ka Shing Centre
    University of Alberta

    Core Manager - Kuni Suzuki kunimasa@ualberta.ca
    Core Director - John Elliott